Merging method for heatmaps

Aim

To specify how data of different tracks are merged in one heatmap.

Procedure

1.Choose Analysis > Results > Plot Heatmaps, or in the Experiment Explorer, under Results, click Heatmap Visualization.

2.In the Heatmap Settings pane, click the Show/Hide tab. Choose one of the options under Merging method.

inset_2000784.jpg 

Cumulated

Each sample in a track gets a weight equal to 1/sample rate. Therefore, the color of a pixel in the heatmap represents the total time that the subject(s) body point was at that location. Choose this option when you want to make a heatmap based on all the possible locations of the animals for a group of tracks. With this method, a subject’s position is over-represented in tracks longer than others.

Mean

Each sample in a track gets a weight equal to 1/track length. The color of a pixel represents the average proportion of a track that is found at that location. Choose this option when you want to make a heatmap that represents the average distribution for that group, and you want to compare averages between two or more groups of tracks.

example  Consider two tracks, recorded at 25 samples/s. One track is 10 samples long, the other 1000 samples long. The total track duration is 1010/25 = 40.4 seconds. A subject’s center point is found at location P two times in track 1 (=20% of track 1), and 100 times in track 2 (=10% of track 2). When the two tracks are merged:

With Cumulated, the color of pixel P represents a total time of (2 x 1/25) + (100 x 1/25) = 4.08 s. This means that, in total, 4.08s / 40.4 s = 10.1% of the data points are at that location. The longer track has a greater weight in this calculation.

With Mean, the color of pixel P represents [(2/10) + (100/1000)]/2 = 0.15 = 15% of the track duration. This means that on average, 15% of the data points are at that location. Short and long tracks have the same weight in this calculation.

Notes

Merging makes sense when two or more tracks share the same value of an independent variable chosen as a header. For example, two tracks with the same of Treatment level = Control. If Control is one of the headers, the two tracks contribute to the same heatmap.